Ribosome RNA Sequencing Services
Are you currently facing challenges in pinpointing microbial contaminants, accurately identifying novel strains, or understanding complex microbial community dynamics in your biopharmaceutical research and development? At Creative Biolabs, our ribosomal RNA sequencing service helps you achieve precise microbial identification and comprehensive community profiling through cutting-edge Next-Generation Sequencing (NGS) technologies and expert bioinformatic analysis.
Overview of Ribosome RNA Sequencing
Ribosome RNA sequencing represents a sophisticated and powerful technique in the field of molecular biology research. This innovative approach involves the sequencing of ribosome-protected mRNA fragments. Through in-depth analysis, it enables researchers to gain profound insights into the intricacies of translational dynamics. It can precisely determine gene translation efficiency, revealing which genes are actively translated and to what extent. Moreover, it aids in the discovery of alternative translation initiation sites, offering a more comprehensive understanding of gene expression regulation at the translational level, thus opening new avenues for biological and medical research.
Fig.1 Ribo-seq analysis of translation in the Ribo-T cells.1
Simplified Workflow
Our streamlined ribosomal RNA sequencing workflow is designed for clarity, efficiency, and robust results, making it easy for you to understand each stage from sample submission to data delivery.
-
Sample Intake & Quality Control (QC)
Upon receiving your samples, we perform initial QC checks to ensure suitability for sequencing. -
Targeted rRNA Gene Amplification
- For identification/metagenomics: Specific regions of rRNA genes (e.g., 16S for bacteria, 18S/ITS for fungi) are amplified using PCR with optimized primer sets.
- For full-length rRNA or modification studies: Ribosomal RNA may be enriched from total RNA if submitted, or specific approaches applied.
-
NGS Library Preparation
The amplified DNA or selected RNA is converted into a sequencing library. This involves fragmenting, adding sequencing adapters, and indexing for multiplexing.
-
Bioinformatics Analysis & InterpretationRaw data undergoes rigorous QC and adapter trimming, and then specialized analysis pipelines are used for taxonomic assignment, diversity analysis, comparative analysis, or variant/modification calling for full-length rRNA.
Key Highlights
- High Accuracy & Sensitivity
Optimized protocols for robust amplification and sequencing of diverse rRNA targets, enabling detection of low-abundance species and subtle variations.
- Comprehensive Bioinformatics
Advanced analytical pipelines for accurate taxonomic assignment, diversity analysis, and, if applicable, rRNA variant/modification detection, providing deeper biological understanding.
- Tailored Solutions
We consult with you to understand your specific project goals and customize our approach, from experimental design to final reporting.
- Fast Turnaround Times
Efficient workflows are designed to deliver your results promptly without compromising quality.
- Dedicated Support
Expert scientific support throughout your project lifecycle.
- Commitment to Quality
Stringent QC measures at every step, ensuring reliable and reproducible data.
Application Areas
- Microbial Contamination Detection & Source Tracking
Rapidly identify and trace bacterial, fungal, or archaeal contaminants in raw materials, cell cultures, bioprocessing lines, and final pharmaceutical products to ensure safety and quality.
- Production Strain Identity Verification & QC
Confirm the identity, purity, and stability of microbial strains used in fermentation, enzyme production, and other biomanufacturing processes.
- Microbiome Analysis in Health & Disease
Characterize complex microbial communities to understand their role in host health, disease pathogenesis, and response to therapeutic interventions.
- Novel Enzyme & Antibiotic Discovery
Screen diverse environmental or biological samples for novel microorganisms that may produce valuable enzymes, antibiotics, or other bioactive compounds.
- Diagnostics & Pathogen Identification
Support the development of diagnostic tools by enabling rapid and accurate identification of pathogenic microorganisms relevant to human or animal health.
- Fermentation Process Optimization
Track microbial community shifts and population dynamics during fermentation processes to optimize yields, product quality, and process stability.
- Assessment of Raw Material Quality
Screen incoming raw materials for microbial load and specific contaminants to prevent downstream issues in production.
FAQs
Q: What types of samples are suitable for your ribosomal RNA sequencing service?
A: We can process a wide range of samples, including purified DNA, bacterial/fungal isolates, complex environmental samples, biological fluids, tissues, and raw materials used in biopharmaceutical production. Contact us to discuss your specific sample type!
Q: How does ribosomal RNA sequencing compare to traditional culture-based microbial identification methods?
A: rRNA sequencing offers significant advantages: it can identify unculturable or difficult-to-culture organisms, provide a comprehensive profile of entire microbial communities, and often deliver results much faster.
Q: What level of taxonomic resolution can I expect?
A: Typically, 16S rRNA sequencing can reliably identify bacteria down to the genus level, and often to the species level, depending on the specific region sequenced and the organisms present. ITS sequencing for fungi usually provides species-level identification.
Q: What are the main precautions to take when preparing samples for rRNA sequencing?
A: To ensure high-quality results, it's crucial to minimize DNA/RNA degradation by using appropriate collection and storage methods. Avoiding cross-contamination between samples is also paramount.
Q: Can you help with interpreting the data and results?
A: Absolutely! Our service includes comprehensive bioinformatics analysis, and our expert team is available to help you understand your results, interpret the findings in the context of your research questions, and discuss potential next steps.
Related Services
To study gene expression profiles of specific organisms or entire communities, often requiring effective rRNA depletion.
Our 16S rRNA sequencing & assembly service is a powerful tool for microbial community analysis. By targeting the 16S rRNA gene, we can identify and classify bacteria and archaea with high precision. Our advanced sequencing technology ensures accurate and reliable results. The assembly process optimizes data utilization, providing detailed insights into microbial diversity, abundance, and taxonomic composition, aiding in various research fields.
- 18S rRNA Sequencing & Assembly Service
The 18S rRNA sequencing & assembly service we offer is tailored for eukaryotic microbial studies. Focusing on the 18S rRNA gene enables the identification and classification of diverse eukaryotic microorganisms. Our cutting-edge sequencing methods and efficient assembly pipelines deliver high-quality data. This service is invaluable for exploring eukaryotic microbial communities, understanding their ecological roles, and advancing related scientific research.
Creative Biolabs is your dedicated partner for advanced ribosomal RNA sequencing services. We provide comprehensive solutions from sample processing to in-depth bioinformatics analysis, empowering researchers in the biopharmaceutical and biochemical fields with accurate microbial identification, detailed community profiling, and critical quality control data. Ready to unlock the microbial dimension of your research? Contact our ribosome technology team to discuss your project.
Reference
- Aleksashin, Nikolay A., et al. "Assembly and functionality of the ribosome with tethered subunits." Nature communications 10.1 (2019): 930. Distributed under Open Access license CC BY 4.0, without modification.
(USA)
(UK)
(Germany)
