Ribosome 16S rRNA Sequencing & Assembly Service
Are you struggling to accurately profile complex microbial communities, facing challenges in achieving species-level resolution, or needing reliable data for your microbiome research? Our 16S ribosomal RNA (rRNA) sequencing & assembly service helps you achieve high-resolution microbial identification, understand community dynamics, and discover novel microbial insights through cutting-edge full-length 16S rRNA gene sequencing, robust assembly algorithms, and advanced bioinformatics pipelines.
Understanding 16S rRNA Sequencing & Assembly
The 16S rRNA gene is a highly conserved yet variable component of the prokaryotic ribosome, essential for bacterial and archaeal life. Its unique structure, featuring both conserved regions and hypervariable regions, makes it an invaluable molecular marker for phylogenetic studies and microbial identification. 16S rRNA sequencing involves targeting and reading the DNA sequence of this gene. Subsequent assembly, especially for full-length sequences, reconstructs the complete gene sequence, allowing for more accurate taxonomic assignment and the differentiation of closely related species within complex microbial communities.
Fig.1 Outline of the rRNA sequencing and annotation procedure.1
Our Streamlined Workflow
Our 16S rRNA Sequencing & Assembly Service is designed for clarity and efficiency, ensuring you receive high-quality data through a transparent process.
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Consultation and Project Design
We begin with a thorough discussion to understand your research goals, sample characteristics and desired analytical depth. This allows us to tailor a project plan, including recommendations for optimal sequencing strategies, such as full-length 16S approaches for enhanced resolution. -
Sample Quality Control (QC) and Library Preparation
Upon receipt, your DNA samples undergo rigorous QC to assess purity and integrity. High-quality DNA then proceeds to library preparation, where the 16S rRNA gene is amplified using optimized primers and prepared for sequencing.
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Advanced Sequencing
Libraries are sequenced using state-of-the-art high-throughput sequencing platforms, potentially including advanced long-read technology to capture full-length 16S rRNA gene sequences. This yields millions of reads, providing deep coverage of your microbial communities.
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Data Processing and Sequence Assembly
Raw sequencing data undergoes stringent quality filtering and trimming. For full-length sequencing projects, sophisticated algorithms are employed for accurate sequence assembly and error correction, generating high-fidelity amplicon sequence variants (ASVs) or operational taxonomic units (OTUs).
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Comprehensive Bioinformatic AnalysisAssembled sequences are assigned taxonomy using curated databases. We perform in-depth analyses including differential abundance analysis and biomarker discovery.
Key Features
- Cutting-Edge Sequencing Technology
Creative Biolabs utilizes state-of-the-art platforms for 16S rRNA sequencing, offering full-length gene amplification to achieve species-level taxonomic resolution. This capability enables precise detection of subtle microbial community shifts critical for mechanistic studies.
- Advanced-Data Accuracy Protocols
Our workflow integrates proprietary error-correction algorithms with optimized bioinformatics pipelines, ensuring reliable ASV analysis. Rigorous quality control minimizes sequencing artifacts and cross-talk between samples.
- End-to-End Scientific Support
From experimental design to biological interpretation, our PhD-level microbiome specialists provide tailored consultation. Services include primer selection strategies, statistical power calculations, and custom visualization of microbiome-disease associations.
- Validated Methodological Excellence
Peer-reviewed publications demonstrate consistent performance across diverse applications, from human cohort studies to environmental metagenomics. Proven track record in resolving complex microbial interactions and biomarker discovery.
Applications
- Human Microbiome Research
Analyzing microbial community structures across gut, oral, skin, vaginal, and respiratory ecosystems. Investigating associations between microbial dysbiosis and diseases. Evaluating dietary, probiotic, and pharmaceutical interventions on microbiome composition and function.
- Environmental Microbiology
Characterizing microbial diversity in terrestrial, aquatic, and atmospheric environments. Tracking pollution/climate impacts on microbial consortia. Identifying keystone species in biogeochemical cycles. Isolating contaminant-degrading microbes for bioremediation applications.
- Agricultural Innovations
Assessing soil microbiome health under different farming practices. Deciphering plant-microbe interactions. Optimizing livestock production through gut microbiome modulation to enhance growth performance and disease resistance.
- Food Industry Applications
Real-time monitoring of microbial dynamics in fermentation processes. Detecting spoilage organisms for food safety assurance. Profiling probiotic strains to validate functional claims and stability.
- Biotechnology Optimization
Controlling microbial consortia in bioreactors for industrial-scale production. Mining metagenomic libraries for novel enzymes/bioactives from uncultured microbes. Implementing microbial contamination testing in pharmaceutical/cosmetic manufacturing.
- Aquatic Ecosystem Management
Using microbial indicators for water quality assessment. Understanding host-microbiome symbiosis in aquaculture species. Establishing microbial surveillance systems to prevent disease outbreaks in aquaculture facilities.
FAQs
Q: What types of samples can Creative Biolabs process for 16S rRNA sequencing?
A: We can process a wide variety of sample types, including but not limited to environmental samples, biological fluids, tissue samples, swabs, food products, and isolated microbial cultures. Please contact us to discuss your specific sample type.
Q: How does full-length 16S sequencing offer an advantage over methods sequencing only variable regions?
A: Full-length 16S rRNA sequencing provides more comprehensive genetic information by covering all nine hypervariable regions. This typically results in higher taxonomic accuracy and resolution, enabling more precise species-level identification and better differentiation of closely related organisms compared to sequencing only one or a few variable regions.
Q: What level of taxonomic resolution can I realistically expect from your service?
A: The achievable taxonomic resolution depends on several factors, including the sample type, microbial diversity, and the specific 16S rRNA gene regions sequenced. Our full-length sequencing services, combined with advanced bioinformatics, aim for species-level resolution where possible, significantly enhancing the depth of your microbial analysis.
Q: How should I prepare and ship my samples to Creative Biolabs?
A: We recommend extracting DNA using a validated method suitable for your sample type to ensure high quality. Once extracted, DNA should be quantified and its purity assessed. We provide detailed guidelines on sample preparation, minimum DNA requirements, and shipping conditions upon project initiation to ensure sample integrity.
Q: My project involves a very large number of samples and unique comparative analyses. Can Creative Biolabs accommodate this?
A: Absolutely. Creative Biolabs is equipped to handle high-throughput projects and offers customizable bioinformatics solutions. We encourage you to discuss your specific needs, project scale, and analytical requirements with our experts so we can develop a tailored strategy that meets your research objectives.
Creative Biolabs is your dedicated partner for advanced 16S rRNA sequencing and assembly. We combine state-of-the-art technology with expert bioinformatic analysis and personalized support to deliver high-resolution microbial community insights, accelerating your discoveries in microbiome research. Ready to advance your microbial research? Contact us now!
Reference
- Rosselli, Riccardo, et al. "Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each taxon." Scientific reports 6.1 (2016): 32165. Distributed under Open Access license CC BY 4.0, without modification.
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