Are you currently facing challenges in isolating high-quality, active ribosomes for your downstream applications, struggling with low yields, or finding it difficult to analyze specific ribosome populations? Our advanced ribosome separation and extraction services at Creative Biolabs help you obtain pure and functionally intact ribosomes for detailed study through cutting-edge biochemical fractionation and affinity purification techniques, accelerating your research in gene expression, drug discovery, and disease mechanisms.
Our key capabilities include the isolation of total ribosomes (70S/80S), ribosomal subunits (30S, 50S, 40S, 60S), polysomes, and specific ribosome populations. We empower your research by providing the essential starting material for ribosome profiling, structural analysis, in vitro translation assays, and the identification of ribosome-associated factors. Our process is meticulously designed for efficiency and to ensure the highest quality ribosome preparations, suitable for a variety of downstream applications.
Sufficient quantity of cryopreserved cells (1x10⁸ mammalian cells) from your specific model system.
A detailed description of your research objectives, the specific type of ribosomes needed (e.g., total ribosomes, polysomes, specific subunits), and any known characteristics of your samples.
If you have specific buffer requirements for your downstream applications, please provide the formulations. Otherwise, we will use our optimized, validated protocols.
This case study details the procedure for preparing HeLa cells and subsequently isolating and purifying ribosomes, divided into sample preparation and ribosome purification stages.
Cells were cultured, and upon reaching confluence, cells were harvested. After low-speed centrifugation, cells were washed twice with PBS, transferred to cryotubes, centrifuged to remove residual liquid, snap-frozen in liquid nitrogen, and stored at -80°C.
Ribosome purification is initiated with ice-cold lysis buffer. The lysates were clarified through centrifugation, following which the supernatants were carefully layered onto sucrose cushions and subjected to ultra-centrifugation to isolate crude ribosomes. Following resuspension, samples underwent pretreated sucrose gradient centrifugation to separate ribosomal fractions. 80S ribosomes were collected, PEG-precipitated, and stored in a resuspension buffer at -80°C.
Fig.1 Ribosome separation profile.
The high-quality ribosome preparations obtained through our specialized services are instrumental for a wide array of cutting-edge research applications. These include:
Isolated ribosomes, particularly polysome fractions or affinity-purified translating ribosomes, are the essential input for Ribo-Seq. This powerful technique allows for genome-wide assessment of protein synthesis by sequencing ribosome-protected mRNA fragments, providing insights into translational control, identifying novel translated regions, and quantifying gene expression at the translational level.
Highly purified and homogenous preparations of ribosomes and their subunits are critical for determining their three-dimensional structures. These structural insights are fundamental for understanding the mechanics of protein synthesis and for rational drug design targeting the ribosome.
Bacterial ribosomes are a major target for antibiotics. Purified prokaryotic ribosomes and their subunits are used in high-throughput screening assays to identify novel antimicrobial compounds that inhibit bacterial protein synthesis.
Advanced separation techniques can help isolate specific subpopulations of ribosomes that may differ in their rRNA modifications, protein composition, or functional state.
Selecting Creative Biolabs for your ribosome separation and extraction needs grants you access to unparalleled expertise and state-of-the-art technology. We are dedicated to delivering solutions that surpass your expectations.
Our team comprises seasoned scientists with extensive experience in ribosome biochemistry, molecular biology, and separation sciences. We understand the nuances of working with these complex ribonucleoprotein particles.
We employ a range of advanced techniques, including optimized sucrose density gradient centrifugation, Ribosome Affinity Purification (RAP), and Translating Ribosome Affinity Purification (TRAP) protocols, ensuring high purity and functional integrity of the isolated ribosomes.
Every ribosome preparation undergoes stringent quality checks, including A260/A280 measurements, RNA integrity analysis, and often, functional validation, to ensure you receive material of the highest standard.
We prioritize efficiency without compromising on quality, striving to deliver your purified ribosomes within agreed-upon timelines to keep your research on track.
You receive detailed reports outlining the entire process, from sample receipt to final QC data, ensuring full transparency and providing valuable insights for your research.
A: We can process a wide variety of samples, including bacterial cultures, yeast, mammalian cell lines, plant tissues, and animal tissues. Our protocols are optimized for diverse source materials to ensure maximum yield and integrity.
A: We employ RNase-free conditions throughout the process, utilize gentle lysis methods, and perform all steps at low temperatures. Our quality control includes RNA integrity checks and A260/A280 purity assessments.
A: Absolutely! We take immense pride in delivering bespoke services tailored to your needs. If you have specific buffer requirements, need to isolate ribosomes from drug-treated cells, or have other particular needs, we will work with you to adapt our protocols accordingly.
A: Sucrose gradient centrifugation is excellent for separating ribosomes based on size (subunits, monosomes, polysomes) and provides a global view of translational activity.
At Creative Biolabs, we are dedicated to empowering your scientific breakthroughs by providing top-tier ribosome separation and extraction services, alongside a comprehensive portfolio of molecular biology solutions. Our expertise ensures you receive high-quality materials and data, accelerating your journey from research to discovery. Ready to learn more about how Creative Biolabs can assist you? Contact our team now!
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