Ribosome Separation and Extraction Services
Isolation of ribosomal particles is critical for studying ribosomal composition and for analyzing factors that interact with ribosomes. As a reputable global service provider, Creative Biolabs has extensive experience in ribosome extraction and isolation experiments. We provide cost-effective services according to the different needs of customers.
Background
Introduction to Ribosome
The ribosome is an important intracellular organelle, which is the molecular machine for intracellular protein synthesis. Ribosomes have no membrane structure and are mainly composed of RNA and proteins. Ribosomes are divided into two categories according to the sedimentation coefficient, one type (the 70S) exists in prokaryotes such as bacteria, and the other type (80S) exists in the cytoplasm of eukaryotic cells. Ribosome extraction refers to the isolation of ribosomes from specific cells or tissues. The prepared ribosomes can be used for the research of ribosome components, ribosome-interacting factors, ribosome-related diseases, and ribosome-related omics.
Strategies for Ribosome Separation
Strategies for isolating ribosomes vary for different organisms and experimental goals. In general, ribosome isolation mainly involves two strategies: centrifugation and immunoprecipitation (IP), both of which have advantages and disadvantages. Centrifugation techniques require long and multiple spins, thus exposing ribosomes to ribonucleases and proteases for extended periods. Another limitation is the poor solubility of the resulting ribosomal particles. In contrast, the IP method is fast and produces soluble ribosome yields. However, it requires adding tags to surface-exposed proteins, which can interfere with the study of the target. In addition, IP methods may lead to undesirable stripping of ribosomal cofactors, affecting study performance.
Fig.1 Work flows of two ribosome
isolation strategies. (Yan, 2015)
Our Service
To obtain high-yield and purity ribosome products to ensure subsequent experimental results, Creative Biolabs has established a ribosome preparation scheme for animal tissue, cultured cells, bacteria, and plant samples to meet your follow-up ribosome composition research and omics analysis goals. We can provide a variety of ribosome isolation services for animal cells, tissues and bacteria, plants, and chloroplasts. Our services include but are not limited to:
- Sucrose Gradient Centrifugation
- Ribosome affinity purification (RAP)
- Translating ribosome affinity purification (TRAP)
Highlights
- Advanced experimental platform and equipment
- The professional research team and rich experience
- Customized ribosome preparation protocols to meet different experimental goals
- Reliable results facilitate follow-up studies
- Short cycles and cost-effective
FAQs
Q1: What should I do if I want to inquire about Creative Biolabs' other ribosome-related services?
A: You can contact our expert team directly. If you have specific and detailed service needs, you can directly request a quote. If not, you can send us a request and we will provide you with a customized solution.
Published Data
Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro
RNA
Authors: Brandon M. Trainor, Dimitri G. Pestov, Natalia Shcherbik
Abstract: Stress-induced molecular damage to ribosomes can impact protein synthesis in cells, but
cell-based assays do not provide a clear way to distinguish the effects of ribosome damage from stress responses
and damage to other parts of the translation machinery. Here we describe a detailed protocol for the separation
of yeast ribosomes from other translational machinery constituents, followed by reconstitution of the
translation mixture in vitro. This technique, which we refer to as ribosome separation and reconstitution (RSR),
allows chemical modifications of yeast ribosomes without compromising other key translational components. In
addition to the characterization of stress-induced ribosome damage, RSR can be applied to a broad range of
experimental problems in studies of yeast translation.
Experimental workflow for ribosome
separation and reconstitution (RSR).
Resource
Ribosome Analysis - Creative Biolabs
Ribosome Analysis - Creative BiolabsReference
- Yan, G.; Yan, X. Ribosomal proteomics: Strategies, approaches, and perspectives. Biochimie. 2015, 113: 69-77.
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