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Ribosomal Transcriptome

Background Our Service Highlights FAQs Published Data Resource

Ribosome is an important machine that is inseparable from many life activities of organisms, such as cell proliferation and protein synthesis. The development of ribosomal transcriptomics has laid the foundation for further understanding of the protein synthesis process. Creative Biolabs is a leading ribosome analysis service provider. At Creative Biolabs, we focus on providing customized service to our global clients, we will help you promote your program to success.

Background

Introduction to Ribosomal Transcriptome

Translation is an essential process in life. It outlines the process of converting genetic information in mRNA nucleotide sequences into proteins. Translation is carried out by the ribosome, which first recognizes the region of the mRNA that encodes the protein, then reads the codons one at a time, assembling a polypeptide whose amino acid sequence corresponds to the codon sequence. Therefore, to understand translation and its regulation, it is essential to determine the location and number of ribosomes on mRNA in vivo. Ribosomal profiling provides an opportunity to explore these locations as a novel strategy. Analysis of the ribosomal transcriptome primarily sequenced cDNA libraries derived from ribosome-covered mRNA short fragments, and ribosome profiling performed transcriptome-wide measurements of ribosome occupancy.

Fig. 1 Ribosome profiles and stop codon read-through mediated changes by Rcm1. (Schosserer, Markus, et al., 2015)Fig. 1 Rcm1 mediates changes in ribosome profiles and stop codon read-through.¹

The Role of Transcriptome Analysis

The transcriptome of the sample provides a more representative expression profile, reducing the search space by eliminating unexpressed gene products. Transcriptome also provides information about sequence variation, such as mutations and editing variations, which can aid in the detection of novel proteins. However, due to extensive translational regulation, the presence of transcripts does not correspond exactly to the presence of the corresponding protein. Recently, a new technique that attempts to address these limitations has been described: ribosome profiling. Breakthroughs in transcriptome analysis have been facilitated by advances in ribosome analysis.

Our Service

We provide flexible analysis services which include but are not limited to:

Highlights

  • Years of experience in ribosome transcriptome analysis services.
  • Advanced technology platform for ribosome purification and analysis.
  • Short cycles and cost-effectiveness.
  • Appropriate and timely after-sales service.

FAQs

Q1: What is ribosomal transcriptome analysis?

A: Ribosomal transcriptome analysis involves sequencing cDNA libraries from ribosome-protected mRNA fragments to study the process of translation. This method helps in understanding how ribosomes select and translate mRNA into proteins, offering insights into protein synthesis and regulation.

Q2: What services does Creative Biolabs offer in ribosome analysis?

A: With years of experience, an advanced technology platform, and a focus on cost-effectiveness and efficient project completion, Creative Biolabs provides a reliable choice for scientists seeking comprehensive ribosome analysis services, including post-analysis support. We offer a range of services including ribosome profiling, polysome profiling, ribosome complex profiling, and ribosome RNA sequencing services. These services are designed to support various research needs related to ribosome analysis.

Q3: Can Creative Biolabs customize their ribosome analysis services?

A: Yes, Creative Biolabs focuses on providing customized ribosome analysis services to meet the specific needs of their global clients. They work closely with researchers to choose the optimal experimental procedures and techniques for each project.

Published Data

Ribosome profiling reveals the rhythmic liver translatome and circadian clock regulation by upstream open reading frames

GENOME RESEARCH

Authors: Peggy Janich, Alaaddin Bulak Arpat, Violeta Castelo-Szekely, et al.

Abstract:

Mammalian gene expression displays widespread circadian oscillations. Rhythmic transcription underlies the core clock mechanism, but it cannot explain numerous observations made at the level of protein rhythmicity. We have used ribosome profiling in mouse liver to measure the translation of mRNAs into protein around the clock and at high temporal and nucleotide resolution. We discovered, transcriptome-wide, extensive rhythms in ribosome occupancy and identified a core set of approximately 150 mRNAs subject to particularly robust daily changes in translation efficiency. Cycling proteins produced from nonoscillating transcripts revealed thus-far-unknown rhythmic regulation associated with specific pathways (notably in iron metabolism, through the rhythmic translation of transcripts containing iron responsive elements), and indicated feedback to the rhythmic transcriptome through novel rhythmic transcription factors. Moreover, estimates of relative levels of core clock protein biosynthesis that we deduced from the data explained known features of the circadian clock better than did mRNA expression alone. Finally, we identified uORF translation as a novel regulatory mechanism within the clock circuitry. Consistent with the occurrence of translated uORFs in several core clock transcripts, loss-of-function of Denr, a known regulator of reinitiation after uORF usage and of ribosome recycling, led to circadian period shortening in cells. In summary, our data offer a framework for understanding the dynamics of translational regulation, circadian gene expression, and metabolic control in a solid mammalian organ.

Transcriptome-wide analysis of transcript abundance and ribosome occupancy rhythms. (Janich, Peggy, et al., 2015) Transcriptome-wide analysis of transcript abundance and ribosome occupancy rhythms. (Janich, Peggy, et al., 2015)

Resource

The success of different experiments depends on appropriate experimental procedures and analytical methods. Scientists in Creative Biolabs will choose the optimal experimental procedures and techniques according to your project needs. Welcome to discuss your ribosome-related project with our experts. Please feel free to contact us for more details.

Reference

  1. Schosserer, Markus, et al. "Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan." Nature communications 6.1 (2015): 6158.
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