Ribosome Analysis Services
As an important macromolecular translation machine in organisms, ribosomes are inseparable from life activities such as protein synthesis and cell proliferation. In recent years, scientists have become more and more interested in the study of ribosome-related factors. As a global reputable ribosome service provider, Creative Biolabs is pleased to provide ribosome analysis services to our customers worldwide.
Background
Ribosomes are complexes of RNA and protein. Ribosomes are large molecular machines that translate sequences of messenger RNA into proteins in all living cells. Furthermore, there is a close correlation between ribosome biogenesis and cell proliferation. There is increasing evidence that dysregulated ribosome biosynthesis is closely associated with an increased risk of cancer and metabolic disorders. Analysis of ribosomes has identified some of the factors involved in these complex processes, providing new perspectives for further in-depth study of ribosome properties and functions.
Our Service
Ribosomal Transcriptome
Both biological sciences and medicine rely on rapid genomic and transcriptomic analysis techniques to probe cellular state, physiology, and activity. Among the technologies, microarray technology and next-generation transcript sequencing (RNA-Seq) are the most advanced. Among them, RNA-Seq offers limitless possibilities in modern biological analysis. For the ribosomal transcriptome, the analysis mainly involves cDNA libraries from short mRNA fragments covered by ribosomes.
Fig.1 A schematic of the ribosome
profiling protocol. (McGlincy, 2017)
Ribosomal Proteomics
In recent years, proteomics research has made great progress with the development of technology. But ribosomal proteomics still lags. Recently, with the rise of the ribosomal filtering hypothesis and ribosome-specific regulatory capabilities, ribosomal proteomics has emerged as an important strategy for understanding the mechanism of ribosome translation. Undoubtedly, the development of ribosomal proteomics provides a promising strategy for explaining the properties and roles of ribosomal proteins.
Fig.2 Work flows of
three strategies for ribosomal proteomic research. (Yan, 2015)
Highlights
- Industry leadership
Our industry-leading expertise in ribosome extraction and analysis can help advance your project.
- Perfect experimental platform
We have established a complete experimental platform for ribosome purification and analysis.
- Professional research team
Our excellent research team specializes in theoretical knowledge and practical skills in ribosome isolation and analysis.
- Abundant experience
We have successfully completed many ribosome projects and accumulated rich experience. We can provide a professionally customized process according to your project needs.
- One-stop service of high quality
Our team can provide diverse and low-cost, high-efficiency one-stop ribosome analysis services.
FAQs
Q1: What techniques are used in analysing the ribosomal transcriptome?
A: The analysis of the ribosomal transcriptome involves genomic and transcriptomic analysis techniques like microarray technology and RNA-Seq. Specifically, RNA-Seq offers limitless possibilities in modern biological analysis. For the ribosomal transcriptome, the analysis primarily involves cDNA libraries from short mRNA fragments covered by ribosomes.
Q2: What capabilities does Creative Biolabs have in the field of ribosome research?
A: Creative Biolabs is a reputable global ribosome service provider that has industry-leading expertise in ribosome extraction and analysis. The company has a complete experimental platform for ribosome purification and analysis and a team specializing in theoretical knowledge and practical skills in ribosome isolation and analysis. We can offer a diverse range of high quality, low-cost, high-efficiency one-stop ribosome analysis services.
Q3: How can I learn more about Creative Biolabs’ additional services related to ribosomes?
A: Feel free to reach out to our team of experts for more information. If you have specific requirements, you can request a quote directly. Otherwise, simply send us your inquiry and we will tailor a solution to meet your needs.
Published Data
Translatome profiling: methods for genome-scale analysis of mRNA translation
BRIEFINGS IN FUNCTIONAL GENOMICS
Authors: Helen A. King, André P. Gerber
Abstract: During the past decade, there has been a rapidly increased appreciation of the role of
translation as a key regulatory node in gene expression. Thereby, the development of methods to infer the
translatome, which refers to the entirety of mRNAs associated with ribosomes for protein synthesis, has
facilitated the discovery of new principles and mechanisms of translation and expanded our view of the
underlying logic of protein synthesis. Here, we review the three main methodologies for translatome analysis,
and we highlight some of the recent discoveries made using each technique. We first discuss polysomal profiling,
a classical technique that involves the separation of mRNAs depending on the number of bound ribosomes using a
sucrose gradient, and which has been combined with global analysis tools such as DNA microarrays or
high-throughput RNA sequencing to identify the RNAs in polysomal fractions. We then introduce ribosomal
profiling, a recently established technique that enables the mapping of ribosomes along mRNAs at near-nucleotide
resolution on a global scale. We finally refer to ribosome affinity purification techniques that are based on
the cell-type–specific expression of tagged ribosomal proteins, allowing the capture of translatomes from
specialized cells in organisms. We discuss the advantages and disadvantages of these three main techniques in
the pursuit of defining the translatome, and we speculate about future developments.
Experimental approaches to study the
translatome.
Resource
Ribosome Analysis - Creative Biolabs
Ribosome Analysis - Creative BiolabsAs a leader in the field of ribosome research, Creative Biolabs aims to provide high-quality ribosome extraction and analysis services to customers around the world. Our customized services will greatly contribute to the success of your project. Please contact us for more information and a detailed quotation.
References
- McGlincy, N. J.; Ingolia, N. T. Transcriptome-wide measurement of translation by ribosome profiling. Methods. 2017, 126: 112-129.
- Yan, G.; Yan, X. Ribosomal proteomics: Strategies, approaches, and perspectives. Biochimie. 2015, 113: 69-77.
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