Tired of mismatches between mRNA and protein levels? Uncover hidden regulatory mechanisms with our advanced polysome profiling Service. By isolating ribosome-bound mRNAs via sucrose gradient ultracentrifugation and integrating multi-omics analysis, we quantify translational efficiency, spotlight regulatory bottlenecks, and reveal biomarkers.
Polysome profiling is a powerful biochemical technique used to isolate and analyze messenger RNAs (mRNAs) based on the number of ribosomes they are associated with. This association directly reflects the translational activity of an mRNA. By separating mRNAs bound to multiple ribosomes (polysomes) from those bound to single ribosomes (monosomes) or no ribosomes, researchers can gain a snapshot of the "translatome"–the entirety of actively translated mRNAs within a cell at a given moment. This method provides crucial insights into gene expression regulation at the translational level, which often complements and extends findings from transcriptomic studies.
Fig.1 Polysome profiling followed by RNA-seq during cardiomyogenic differentiation.1
To guarantee optimal results, kindly follow the sample requirements outlined below:
Adherent cells: ≥1×10⁷ cells (harvested at 80–90% confluency).
Suspension cells: ≥1×10⁷ cells (viability >90% recommended).
Yeast: ≥1×10⁸ cells (log-phase growth preferred).
Tissue samples: ≥100 mg (snap-frozen in liquid nitrogen).
Samples must be preserved in RNA-stable reagents or frozen immediately at -80°C. Ship on dry ice with insulated packaging to prevent thawing. For sample preparation details beyond these specifications (e.g., specialized lysis buffers, treatment protocols, or rare sample types), contact our technical team for tailored guidance before submission.
Our polysome profiling workflow is meticulously designed to ensure high-quality data and actionable insights. Key steps involved:
1. Comprehensive Project Report
Including detailed methodology, raw and processed data (e.g., polysome profiles, sequencing reads), bioinformatic analysis results, and interpretation of findings.
2. High-Resolution Figures
Publication-quality visuals summarizing key results, such as polysome profile traces, heatmaps of differentially translated genes, and gene set enrichment plots.
3. Processed Data Files
Including gene expression tables, translational efficiency scores, and lists of significantly altered genes.
This case study employed polysome profiling to analyze and compare ribosomal components across distinct experimental conditions, unveiling crucial insights into translational dynamics.
![]() Fig.2 Ribosome fraction separation diagram. |
![]() Fig.3 Comparison of translation efficiency. |
![]() Fig.4 Differentially expressed analysis. |
![]() Fig.5 Correlation analysis. |
![]() Fig.6 GO enrichment. |
![]() Fig.7 KEGG enrichment bubble chart. |
Our polysome profiling service offers critical insights across a diverse range of research fields by directly measuring the translational status of genes. Key applications include:
Partner with specialists who bridge transcriptomics and proteomics. We design experiments to capture active translation, ensuring your data reflects functional gene expression, not just mRNA levels.
Access state-of-the-art polysome isolation and sequencing (Polysome-Seq). Paired with optimized protocols, we deliver robust data across diverse samples, from human tissues to microbial cultures.
From experimental design to advanced bioinformatics, our team provides seamless guidance. We decode translational efficiency metrics and regulatory mechanisms, aligning findings with your research goals.
Benefit from rigorous QC and standardized workflows. Our data directly answers your biological questions, backed by a proven track record in high-impact journals.
A: Our standard protocol utilizes a 10%-45% sucrose density gradient for ultracentrifugation, yielding 18 fractions to capture monosomes, polysomes, and free mRNA subpopulations. This approach ensures high-resolution separation of ribosome complexes. For projects requiring specialized optimization, such as gradient range adjustments or fraction consolidation, we provide fully customizable workflows aligned with your specific biological targets.
A: Our service works with various samples, like cultured cells, tissues from different organisms, yeast, and bacteria. The main thing is proper collection and preservation to keep polysomes intact. We offer tailored sample-prep guidance.
A: Its key advantage is directly assessing translational efficiency. It helps identify genes regulated at the protein-synthesis level, uncover translational control mechanisms, and understand how cells adjust protein production quickly in response to stimuli without changing mRNA levels.
A: From sample receipt to delivery of your final report, the service usually takes 6 to 10 weeks. However, the exact timeline may vary based on factors such as the number of samples, the complexity of your experimental design, the depth of sequencing chosen, and any specialized bioinformatics analyses required. We will provide a detailed project timeline upfront and keep you updated as your study progresses.
A: Standard RNA-Seq measures total mRNA abundance, which may not match protein levels. Polysome profiling, especially with sequencing (Polysome-Seq), quantifies mRNAs actively being translated. This gives a more direct measure of gene expression at the protein-synthesis level, highlighting key regulatory steps transcriptomics misses. We can advise on the best approach or combo for your research.
For high-resolution mapping of ribosome positions on mRNA, providing detailed insights into translation initiation sites, elongation dynamics, and novel ORFs.
Comprehensive transcriptome analysis, including mRNA, total RNA, small RNA, and long non-coding RNA sequencing, to correlate translational data with overall transcript abundance.
Directly measure protein abundance using mass spectrometry-based approaches to validate and complement findings from translatome studies.
Creative Biolabs is committed to providing cutting-edge polysome profiling services that empower researchers to unravel the complexities of translational regulation. Discover how we can accelerate your research, request a consultation today.
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