Ribosome Complex Profiling

Creative Biolabs is a leading service provider that focuses on ribosomal research. Based on our advanced platforms and extensive experience, now we provide a novel ribosome complex profiling service for our clients all over the world.

GTI-Seq Technology in Ribosome Complex Profiling

Composed of ribosomal RNA molecules and proteins, ribosomes are important tools for protein synthesis in cells. Ribosome complex profiling provides information on all ribosomes active in the cell at a specific time point and helps determine which proteins are actively translated in the cell. In life activities, mRNA translation is a key link that represents the flow of genetic information as well as defines the proteome. As an essential step in gene expression, the translation process plays important role in multiple developmental and physiological processes. To better understand translational regulation, it is important to elucidate the mechanism and regulation of translation initiation (TI).

The global translation initiation sequencing (GTI-Seq) technology has been developed to identify TIS at nucleotide resolution. The GTI-seq technology is based on the translation inhibitor lactimidomycin (LTM), which completely freezes the initiating ribosome. Different from traditional translation inhibitor cycloheximide, LTM acts preferentially on the initiation ribosome and the ribosomal footprint associated with LTM is highly enriched at annotated initiation codons. Importantly, GTI-Seq uses a simple computational approach that minimizes bias when mapping global TIS sites. To facilitate mechanistic studies of alternative translation of individual genes, a comprehensive TIS database (TISdb) based on multiple high-resolution GTI-Seq datasets has been designed.

Fig.1 Schematic diagram of the experimental design for GTI-seq. (Calvo, 2009)

Advantages and Disadvantages of GTI-Seq Technology

GTI-seq, as a new technology capable of mapping global mRNA translation initiation, has the potential to reveal the hidden coding potential of the transcriptome. The study of translational control can further improve our understanding of the diversity and complexity of the proteome. However, there are also a series of limitations. The current TISdb cannot provide the efficiency of alternative translation initiation due to the lack of quantitative capabilities of current ribosome profiling methods. For genes with relatively high expression, GTI-seq may not capture some TIS signals with lower initial efficiency. In conclusion, the integration of GTI-seq technology with other technologies will provide a new perspective on global gene regulation.

Features of Our Ribosome Complex Profiling Service

• Advanced platform
• Well-trained scientists and extensive experience
• Low price and high-quality service
• Customized service based on the standard process to meet different customer needs

Creative Biolabs has been a long-term expert in the field of ribosome research. Based on our extensive experience and advanced platforms, we are confident in offering a series of ribosome-related services, including ribosome separation and extraction services, ribosome analysis services, as well as ribosomal marker antibody development services. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.

Reference

1. Calvo, S.; et al. Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans. Proceedings of the National Academy of Sciences. 2009, 106(18): 7507-7512.