Ribosome Complex Profiling Service
Creative Biolabs is a leading service provider that focuses on ribosomal research. Based on our advanced platforms and extensive experience, now we provide a novel ribosome complex profiling service for our clients all over the world.
Background
Composed of ribosomal RNA molecules and proteins, ribosomes are important tools for protein synthesis in cells. Ribosome complex profiling provides information on all ribosomes active in the cell at a specific time point and helps determine which proteins are actively translated in the cell. In life activities, mRNA translation is a key link that represents the flow of genetic information as well as defines the proteome. As an essential step in gene expression, the translation process plays important role in multiple developmental and physiological processes. To better understand translational regulation, it is important to elucidate the mechanism and regulation of translation initiation (TI).
The global translation initiation sequencing (GTI-Seq) technology has been developed to identify TIS at nucleotide resolution. The GTI-seq technology is based on the translation inhibitor lactimidomycin (LTM), which completely freezes the initiating ribosome. Different from traditional translation inhibitor cycloheximide, LTM acts preferentially on the initiation ribosome and the ribosomal footprint associated with LTM is highly enriched at annotated initiation codons. Importantly, GTI-Seq uses a simple computational approach that minimizes bias when mapping global TIS sites. To facilitate mechanistic studies of alternative translation of individual genes, a comprehensive TIS database (TISdb) based on multiple high-resolution GTI-Seq datasets has been designed.
Fig. 1 Biological processes and functional pathways enriched in the differentially translated genes identified by different methods.¹
Our Service
Creative Biolabs delivers comprehensive ribosome complex profiling services, including:
- Ribosome Isolation and Extraction: We use optimized protocols for precise isolation of ribosome complexes from different biological samples, including eukaryotic and prokaryotic systems.
- Complex Characterization: Employing mass spectrometry and cryo-electron microscopy (cryo-EM), we provide detailed structural and compositional analysis of ribosomal complexes.
- Functional Assays: We offer functional studies that include translation efficiency assessments and analysis of ribosomal pausing, enabling the discovery of how ribosomal complexes regulate protein synthesis.
Highlights
- Extensive Expertise
Over 20 years of specialized experience in ribosome profiling, supported by a highly skilled scientific team.
- Comprehensive Analysis
From isolation to detailed characterization and functional assays, we provide a full suite of services to investigate ribosomal complexes.
- Advanced Technology
Utilizing the latest tools in mass spectrometry, cryo-EM, and next-generation sequencing (NGS), we ensure precise, high-resolution insights into ribosome complex behavior.
- Customizable Solutions
Our protocols are tailored to meet the specific needs of your project, allowing for flexibility in handling various sample types and research goals.
- Reliable Data
Rigorous quality control ensures that every dataset is accurate and reproducible, backed by thorough analysis from our expert team.
- Cost-Effective
We offer competitive pricing without compromising on the quality and precision of our services.
- Dedicated Support
Our expert team provides ongoing support throughout your research journey, from consultation to final interpretation, ensuring your project's success.
FAQs
Q1: What types of samples can be used for ribosome complex profiling?
A: We accept a wide range of samples, including cells from prokaryotes, eukaryotes, and specialized tissues. Our team will advise you on the best sample preparation methods for your specific research needs.
Q2: Can I customize the service to focus on specific ribosomal components?
A: Yes, we offer highly customizable options to target specific ribosomal components or interaction partners, ensuring that the service aligns with your research objectives.
Q3: What output can I expect from the service?
A: The typical polysome profiling procedure involves several key steps: preparation of cell lysates, creation of sucrose gradients, isolation of polysomes, RNA isolation followed by RT-qPCR, and protein isolation using SDS-PAGE. These steps enable a comprehensive analysis of translation activity in mRNAs.
Q4: What are the key advantages of ribosome complex profiling?
A: Ribosome complex profiling provides detailed insights into the structure and function of ribosomes, enabling researchers to study translation dynamics, co-translational processes, and protein synthesis regulation in a wide range of organisms and conditions.
Q5: What types of ribosome-related research can benefit from this service?
A: This service is valuable for studying translation control, ribosome biogenesis, drug interactions targeting ribosomal functions, and protein synthesis regulation under stress or disease conditions.
Resource
Ribosome Analysis - Creative Biolabs
Ribosome Analysis - Creative BiolabsCreative Biolabs has been a long-term expert in the field of ribosome research. Based on our extensive experience and advanced platforms, we are confident in offering a series of ribosome-related services, including ribosome separation and extraction services, ribosome analysis services, as well as ribosomal marker antibody development services. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.
Reference
- Xiao, Zhengtao, et al. "Genome-wide assessment of differential translations with ribosome profiling data." Nature communications 7.1 (2016): 11194.
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