Are you currently struggling to identify truly translated mRNA targets, uncertain about the functional impact of mRNA isoforms, or needing to pinpoint changes in protein synthesis under specific conditions? At Creative Biolabs, our ribosome affinity purification (RAP) service helps you accurately identify and quantify actively translated mRNAs, enabling you to gain deeper insights into gene regulation at the translational level through our optimized RAP protocols, advanced affinity purification strategies, and comprehensive downstream analysis capabilities.
Our RAP service is meticulously engineered to provide an encompassing and completely transparent journey. We'll be your dedicated companion, guiding you seamlessly from the moment you submit your initial samples right up to the point where you have actionable data in hand. Below are the pivotal steps that make up this process:
In complex tissues with various cell types, RAP Service enables targeted ribosome purification from specific populations. This is done by expressing ribosome-binding affinity tags in a cell-type-specific way. Analyzing the mRNAs on these isolated ribosomes reveals each cell type's unique translational profile, uncovering actively translated genes and their regulatory mechanisms.
During development, cells show dynamic gene expression and protein synthesis changes. RAP Service tracks translational alterations at different stages. Isolating ribosomes from developing tissues/organs helps identify mRNAs preferentially translated in processes like organogenesis, cell differentiation, and morphogenesis, aiding the understanding of developmental transitions and translational control.
Abnormal protein synthesis is common in diseases. RAP Service compares translational profiles between healthy and diseased cells/tissues. Identifying differentially translated mRNAs in disease states points to potential therapeutic targets and disease-specific biomarkers.
Cells adjust gene expression at the translational level under environmental stresses like heat shock, oxidative stress, or nutrient deprivation. RAP Service captures translational changes under stress. Isolating ribosomes from stressed cells and analyzing associated mRNAs identifies up or down-regulated genes, revealing cellular stress-coping strategies and molecular mechanisms.
The brain's complexity, with diverse neuronal types and circuits, makes RAP Service valuable. It studies translational regulation in specific neuronal populations, aiding the understanding of neural functions, synaptic plasticity, and neurological disorders. Isolating ribosomes from different brain regions or neuronal subtypes reveals genes critical for neural development, memory formation, and neurodegenerative disease pathogenesis.
Creative Biolabs is steadfastly dedicated to offering state-of-the-art RAP services that yield highly reliable and profoundly insightful data. Our entire methodology rests on a solid bedrock of scientific prowess and an unwavering commitment to top-notch quality.
We utilize optimized protocols, including the potential use of advanced reagents for tagged ribosome immunoprecipitation, to ensure highly specific capture of translating ribosomes and minimize co-purification of non-ribosomal contaminants.
Our team has experience processing a variety of biological samples, including mammalian cells, yeast, and bacteria, adapting protocols to suit specific experimental systems.
We offer a suite of downstream analysis services, from RT-qPCR to full RNA sequencing and bioinformatics, providing a seamless workflow from sample to publication-ready data.
Creative Biolabs offers flexible service options, from providing purified ribosome-associated RNA to full end-to-end analysis, tailored to your specific needs and budget.
We ensure clear and consistent communication at every stage of your project, delivering regular updates and comprehensive reports.
A: RAP isolates full-length mRNAs associated with ribosomes, giving you information about which mRNAs are being translated. Ribo-seq, on the other hand, sequences only the ribosome-protected mRNA fragments, providing positional information about ribosomes on the mRNA. Both are formidable, the best choice depends on your research question. We can help you decide which combination is best for your project.
A: RAP is primarily designed to capture mRNAs actively engaged with ribosomes. While some non-coding RNAs might associate with ribosomes in regulatory roles, our protocols are optimized to enrich for translating mRNAs.
A: Our capabilities allow us to isolate ribosomes from an extensive array of species. This includes human, yeast, bacterial, and plant samples. Should you require more detailed information regarding specific species, please get in touch with us.
A: We implement multiple QC steps, including validation of tagged protein expression, assessment of RNA integrity post-purification, and checks for genomic DNA contamination.
A: Our service offers access to optimized protocols, specialized reagents, experienced personnel, and dedicated instrumentation, potentially saving you significant time and resources in establishing and troubleshooting the technique.
Ready to see how we can streamline your research? Get in touch with us for further details.
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