Translating Ribosome Affinity Purification (TRAP) Service

Creative Biolabs is the world's leading provider of ribosome analysis services. With our extensive experience in ribosome research, we are proud to offer our clients translational ribosome affinity purification (TRAP) services at the highest quality and most competitive prices.

Background

In recent years, the analysis of RNA transcript levels by different techniques such as microarray and RNA-seq has contributed to the advancement of biology. However, RNA expression profiles present some limitations in studying tissues composed of multiple cell types. Therefore, TRAP has been proposed as a promising strategy that allows the isolation of transcripts from intact tissues of selected cell types without dissociation.

TRAP plays an important role in the isolation of ribosome-bound mRNA from specific cell populations from tissues. By epitope tagging of ribosomal proteins in a cell-type-specific manner, polysomes can be selectively purified without laborious cell dissociation and sorting steps. In addition, TRAP provides the closest approximation to cell-type-specific gene expression profiles in intact tissues, since gene expression does not continue after tissue lysis, and the translationome is more representative of the steady-state proteome than the transcriptome.

Fig. 1 Ribosomes are overproduced in hippocampal neurons.¹

Our Service

Creative Biolabs offers a highly specialized TRAP service, leveraging our deep expertise in ribosome biology and molecular analysis. Our TRAP platform is designed to facilitate the isolation of translating mRNA from selected cell populations in intact tissues, enabling researchers to explore gene expression and protein synthesis with high precision. By introducing an enhanced GFP (EGFP) tag to the ribosomal protein L10a, we ensure the selective purification of polysomes from targeted cells. The process involves lysing the cells, capturing EGFP-labeled polysomes using an anti-GFP affinity matrix, and isolating the ribosome-bound mRNA for downstream analysis, including RNA sequencing, microarray, qPCR, or other genomic techniques. This service is ideal for researchers seeking to understand cell-type-specific translational profiles in complex tissues, offering unparalleled sensitivity and specificity. With a highly experienced team and state-of-the-art technologies, Creative Biolabs is committed to delivering tailored TRAP solutions that align with your research objectives.

Highlights

• Cell-Type-Specific Precision

TRAP enables the isolation of ribosome-bound mRNA from specific cell types without the need for tissue dissociation, preserving the in vivo context.

• High Sensitivity

Compared to traditional mRNA analysis techniques, TRAP offers greater sensitivity in detecting low-abundance mRNAs and provides a more accurate representation of the translation landscape.

• Comprehensive Data

TRAP captures active translation in the target cell population, offering insights that go beyond transcriptional profiles by providing data on mRNA that is actively being translated into protein.

• Versatile Applications

This method is compatible with various downstream analyses such as RNA sequencing, microarrays, and qPCR, allowing for comprehensive exploration of translational control mechanisms.

FAQs

Resource

Ribosome Analysis - Creative Biolabs Ribosome Analysis - Creative Biolabs

Creative Biolabs is committed to providing ribosome analysis services. Our services include not only ribosome extraction and isolation, but also analysis of ribosome structure and associated factors. If you have any questions about ribosome-related projects, please contact us in time.

Reference

1. Seo, Sang S., et al. "Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome." Nature Communications 13.1 (2022): 3236.