Ribosomal Proteomics

Introduction of Ribosomal Proteomics

In the past decades, proteomic research has seen unprecedented development due to the emergence of powerful technologies. Advances in ribosomal proteomics have been largely limited by the traditional perception that a ribosome is merely a translation machine. With the proposal of the ribosome filtering hypothesis and the study of ribosome-specific regulation ability, ribosomal proteomics has become a powerful tool for ribosome research.

It is well-known that gene expression can be regulated by multiple cellular mechanisms, such as RNA processing, transcription, translation, as well as protein degradation. Among them, translation is the process of polypeptide synthesis using the information encoded in mRNA, and control of the translation process is the primary mechanism for regulating eukaryotic gene expression. In nature, the ribosomal proteome can tightly regulate the translation process, so the study of ribosomal proteomics is crucial for understanding the translation mechanism of ribosomes.

Fig.1 Schematic showing the drawbacks of whole-cell proteomics compared to subcellular proteomics. (Yan, 2015)

Strategies for Ribosomal Proteome Identification

To better separate and identify proteins for ribosomal proteomic research, a series of advanced MS techniques have been developed. Creative Biolabs provides both label-dependent strategies and label-independent strategies. Among them, stable isotope labeling by amino acids in cell culture (SILAC), isobaric tags for relative and absolute quantitation(iTRAQ), and isotope-coded affinity tag (ICAT) are the most frequently used label-dependent strategies. While coupling RFHR 2-DE with MALDI-TOF MS and multiple reaction monitoring (MRM) are the classical label-independent strategies.

Ribosomal Protein Expression and Analysis

In eukaryotes, there are three types of the ribosome which include cytosolic, mitochondrial, and chloroplast. A number of ribosome-associated proteins (RAPs) can bind to ribosomes, and together with ribosomal proteins (RPs) make up the ribosomal proteome. Creative Biogene has built a complete ribosomal protein expression technology platform to provide a one-stop protein expression service including plasmid construction, gene expression, protein purification, protein expression, and so on. Furthermore, we can also provide the ribosome-interacting proteins analysis for ribosomal proteomics research.

Advantages of our Services

• Extensive experience in ribosomal proteomics analysis
• Customized service based on standard process
• Reliable data and results
• Rapid turnaround and cost-effective

Creative Biolabs has been a long-term expert in the field of ribosome research. Based on our extensive experience and advanced platforms, we are confident in offering a series of ribosome-related services, including ribosome separation and extraction services, ribosome analysis services, as well as ribosomal marker antibody development services. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.

Reference

1. Yan, G.; Yan, X. Ribosomal proteomics: Strategies, approaches, and perspectives. Biochimie. 2015, 113: 69-77.