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Ribosomal Proteomics

Background Our Service Highlights FAQs Published Data Resource

Ribosome is an important machine that is inseparable from many life activities of organisms, such as cell proliferation and protein synthesis. The development of ribosomal transcriptomics has laid the foundation for further understanding of the protein synthesis process. Creative Biolabs is a leading ribosome analysis service provider. At Creative Biolabs, we focus on providing customized service to our global clients, we will help you promote your program to success.

Background

Introduction of Ribosomal Proteomics

In the past decades, proteomic research has seen unprecedented development due to the emergence of powerful technologies. Advances in ribosomal proteomics have been largely limited by the traditional perception that a ribosome is merely a translation machine. With the proposal of the ribosome filtering hypothesis and the study of ribosome-specific regulation ability, ribosomal proteomics has become a powerful tool for ribosome research.

It is well-known that gene expression can be regulated by multiple cellular mechanisms, such as RNA processing, transcription, translation, as well as protein degradation. Among them, translation is the process of polypeptide synthesis using the information encoded in mRNA, and control of the translation process is the primary mechanism for regulating eukaryotic gene expression. In nature, the ribosomal proteome can tightly regulate the translation process, so the study of ribosomal proteomics is crucial for understanding the translation mechanism of ribosomes.

Fig. 1 Dissection and characterization of ribosomal particles (Van de Waterbeemd, Michiel, et al., 2018)Fig. 1 Three-tiered mass spectrometry based approach for the dissection and characterization of ribosomal particles.¹

Our Service

Strategies for Ribosomal Proteome Identification

To better separate and identify proteins for ribosomal proteomic research, a series of advanced MS techniques have been developed. Creative Biolabs provides both label-dependent strategies and label-independent strategies. Among them, stable isotope labeling by amino acids in cell culture (SILAC), isobaric tags for relative and absolute quantitation(iTRAQ), and isotope-coded affinity tag (ICAT) are the most frequently used label-dependent strategies. While coupling RFHR 2-DE with MALDI-TOF MS and multiple reaction monitoring (MRM) are the classical label-independent strategies.

Ribosomal Protein Expression and Analysis

In eukaryotes, there are three types of the ribosome which include cytosolic, mitochondrial, and chloroplast. A number of ribosome-associated proteins (RAPs) can bind to ribosomes, and together with ribosomal proteins (RPs) make up the ribosomal proteome. Creative Biogene has built a complete ribosomal protein expression technology platform to provide a one-stop protein expression service including plasmid construction, gene expression, protein purification, protein expression, and so on. Furthermore, we can also provide the ribosome-interacting proteins analysis for ribosomal proteomics research.

Highlights

  • Extensive experience in ribosomal proteomics analysis
  • Customized service based on standard process
  • Reliable data and results
  • Rapid turnaround and cost-effective

FAQs

Q1: What is ribosomal proteomics and why is its study crucial?

A: Ribosomal proteomics is a science that focuses on the proteomes of ribosomes - the cellular organelles responsible for protein synthesis. Its study is crucial because changes in the ribosomal proteome can regulate translation, a primary method of gene expression regulation, thereby significantly impacting cell functions.

Q2: How does Creative Biolabs approach ribosomal proteome Identification?

A: Creative Biolabs utilizes both label-dependent and label-independent strategies. These include stable isotope labeling by amino acids in cell culture (SILAC), isobaric tags for relative and absolute quantitation (iTRAQ), and isotope-coded affinity tag (ICAT), along with other state of the art Mass Spectrometry (MS) techniques.

Q3: What other ribosome-related services does Creative Biolabs offer?

A: In addition to ribosome separation and analysis services, Creative Biolabs also offers ribosomal marker antibody development services. We are a long-term expert in the field, capable of providing extensive services for ribosome-related research.

Published Dat

A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production

NATURE COMMUNICATIONS

Authors: Naomi R. Genuth, Zhen Shi, Koshi Kunimoto, et al.

Abstract:

Recent findings suggest that the ribosome itself modulates gene expression. However, whether ribosomes change composition across cell types or control cell fate remains unknown. Here, employing quantitative mass spectrometry during human embryonic stem cell differentiation, we identify dozens of ribosome composition changes underlying cell fate specification. We observe upregulation of RPL10A/uL1-containing ribosomes in the primitive streak followed by progressive decreases during mesoderm differentiation. An Rpl10a loss-of-function allele in mice causes striking early mesodermal phenotypes, including posterior trunk truncations, and inhibits paraxial mesoderm production in culture. Ribosome profiling in Rpl10a loss-of-function mice reveals decreased translation of mesoderm regulators, including Wnt pathway mRNAs, which are also enriched on RPL10A/uL1-containing ribosomes. We further show that RPL10A/uL1 regulates canonical and non-canonical Wnt signaling during stem cell differentiation and in the developing embryo. These findings reveal unexpected ribosome composition modularity that controls differentiation and development through the specialized translation of key signaling networks.

Quantitative proteomics reveals ribosome composition changes during hESC differentiation. (Genuth, Naomi R., et al., 2022)Quantitative proteomics reveals ribosome composition changes during hESC differentiation. (Genuth, Naomi R., et al., 2022)

Resource

Creative Biolabs has been a long-term expert in the field of ribosome research. Based on our extensive experience and advanced platforms, we are confident in offering a series of ribosome-related services, including ribosome separation and extraction services, ribosome analysis services, as well as ribosomal marker antibody development services. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.

Reference

  1. Van de Waterbeemd, Michiel, et al. "Dissecting ribosomal particles throughout the kingdoms of life using advanced hybrid mass spectrometry methods." Nature communications 9.1 (2018): 2493.
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