Ribosomal Protein Expression Service
Creative Biolabs is a leading service provider that focuses on ribosomal proteomics research. Based on our advanced platforms and extensive experience, now we can provide the ribosomal protein expression service based on various expression systems.
Background
Ribosomal proteins are integral components of the ribosome, which orchestrates protein translation. Increasing findings suggest that ribosomal proteins, being abundant cellular components, are involved in various regulatory and pathological processes beyond their traditional role in translation. Understanding and leveraging the properties of ribosomal proteins requires precise expression systems tailored to specific research needs, underscoring the importance of robust and versatile ribosomal protein expression services.
Fig. 1 Ribosomal protein uS7 is the major binding protein of fucoxanthinol.¹
Our Service
Creative Biolabs offers a comprehensive ribosomal protein expression service, providing tailored solutions to produce high-quality ribosomal proteins essential for diverse research applications. Leveraging advanced platforms and an experienced team, we ensure optimized workflows that include gene design, codon optimization, expression condition testing, and protein purification. Our service is customizable to fit specific experimental needs and achieve reliable, reproducible results.
Expression Systems Offered
- E. coli Expression System
The most widely used prokaryotic expression system, valued for its short culture period, clear genetic background, and robust contamination resistance. Our engineered strains guarantee high yields, exceptional purity, and controlled endotoxin levels, making them ideal for large-scale ribosomal protein production.
- Yeast Expression System
A versatile platform for expressing recombinant eukaryotic proteins with improved solubility and scalability. This cost-effective system supports large-scale fermentation, ensuring reliable production of ribosomal proteins optimized for various applications.
- Mammalian Cell Expression System
Using CHO and HEK 293 cells, this system ensures ribosomal proteins with post-translational modifications closest to their natural states. Additional capabilities include label removal, endotoxin removal, and serum-free suspension culture for specialized research requirements.
- Baculovirus Expression Vector System
An efficient method for producing ribosomal proteins in insect cells, leveraging codon optimization and the biphasic life cycle of baculoviruses. This system supports high-yield and high-quality expression for applications requiring insect cell specificity.
Service Process
- Gene Design and Codon Optimization
Comprehensive design of expression constructs with optimized codon usage tailored to the selected expression system. This step maximizes translation efficiency and protein yield.
- Expression Condition Testing
Screening of 3-5 expression systems under 2-3 temperature conditions to identify the optimal combination for protein production.
- Protein Expression Scaling
Protein expression is scaled based on client requirements, with precise yield predictions to meet experimental or production needs.
- Protein Purification
Purification processes ensure a purity level exceeding 90%, ensuring proteins are suitable for high-sensitivity applications.
Highlights
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Multiple Expression Systems
A range of expression platforms provides flexibility to select the system best suited to experimental objectives. -
Customizable Workflows
Each project is designed to meet specific requirements, from small-scale experimental needs to large-scale protein production. -
Cost-effectiveness and High Quality
Affordable services with no compromise on quality ensure optimal value for clients. -
High Purity and Safety Standards
With endotoxin levels as low as 1 EU/μg and protein purity above 90%, our products meet the highest standards for research and experimental needs. -
Rapid Turnaround Time
Our streamlined workflows enable efficient project completion, delivering results promptly to accelerate your research.
FAQs
Q1: Can Creative Biolabs customize protein expression conditions?
A: Yes, we customize expression conditions, including the choice of system, temperature, and culture conditions, to ensure optimal production and functionality of the ribosomal proteins.
Q2: How does Creative Biolabs address scalability for ribosomal protein production?
A: We provide precise yield predictions and scalable expression systems tailored to the client's requirements, from small-scale experiments to large-scale production.
Q3: When should the mammalian cell expression system be used?
A: The mammalian system is ideal for producing ribosomal proteins that require post-translational modifications, such as glycosylation, that closely mimic natural states.
Resource
Ribosome Analysis - Creative Biolabs
Ribosome Analysis - Creative BiolabsIn addition to ribosomal protein expression, Creative Biolabs offers a range of ribosome-related services, including ribosome separation and extraction, ribosome analysis, and ribosomal marker antibody development. If you are interested in our ribosomal protein expression service or any of our other ribosome-focused solutions, please contact us for more details. We look forward to supporting your research endeavors with reliable, high-quality services.
Reference
- Iizumi, Yosuke, et al. "Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol." Communications Biology 5.1 (2022): 564. Distributed under the Open Access license CC BY 4.0, without modification.
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