Ribosomal Protein Abundance Analysis Service

Ribosomes are essential components of the protein synthesis machinery. The process of ribosome biogenesis is well organized and tightly regulated. Therefore, any dysregulation of this process may result in aberrant cell proliferation and clinical manifestations of pathological states, such as cancer and metabolic disorders. Due to the development of proteomics techniques, relative abundance analysis of ribosomal proteins provides a holistic approach to explaining the properties, functions, and distribution of ribosomal proteins, while making the ribosome a research hot spot, and ribosomal proteomics began to attract considerable attention.

As an advanced biotech company, Creative Biolabs has launched comprehensive ribosomal proteins relative abundance analysis services to solve your problems in related research. Based on our established protein analysis platforms, our customized proteomics services have achieved remarkable goals in disease diagnosis, pathological research, and drug discovery. Thus, we are confident in providing you with the best services.

Introduction to Relative Abundance of Ribosomal Proteins

The synthesis of the ribosome is a highly ordered cellular process that requires a substantial expenditure of energy. Under stress situations, with decreased ribosome activity, protein synthesis was reduced, and subsequent growth was arrested. Thus, the relative abundance of ribosomal proteins reflects the rate of ribosome biogenesis, which is a critical element involved in controlling cell growth and proliferation. Several human diseases have been demonstrated to be associated with defects in ribosome biogenesis, including increased cancer susceptibility. Therefore, the relative abundance of ribosomal proteins has been regarded as a potential biomarker for many related diseases.

Fig. 1 Some RPs rapidly incorporate into neuronal ribosomes.¹

Methods of Relative Abundance Analysis of Ribosomal Proteins

There are two main approaches to processing and analyzing Mass spectrometry data to quantify and statistically compare protein relative abundances: the first is based on spectral data generated during the process of identification, while the second makes use of extracted ion currents to quantify chromatographic peaks and infer protein abundances based on peptide quantification. With the development of proteomic analysis, multiple strategies are applied in ribosomal proteomic research. In 2014, scientists have quantified the relative abundance of the bacterial ribosome proteins using specific technology, and flexible tools for the quantification of the components of ribosomal protein synthesis machinery were developed. Moreover, because of its central importance in cellular function, the ribosome is the target for many classes of antibacterial agents. Therefore, the convenient method to measure the relative abundance of ribosomal proteins is useful in novel antibiotic screening.

Fig. 2 RP mRNAs are present in hippocampal dendrites.¹

It is no doubt that ribosomal proteins are promising in diagnosis and treatment, while the relative abundance analysis of ribosomal proteins laid the basis of ribosomal proteomics analysis. Creative Biolabs offers our clients rapid and inexpensive Relative Abundance of Ribosomal Proteins Analysis service. We are confident in presenting a comprehensive set of services to explain the secrets of ribosome proteins to worldwide clients. For more details of our service, please feel free to contact us to assist you.

Reference

1. Fusco, Claudia M., et al. "Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins." Nature communications 12.1 (2021): 6127.