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Quantification of Ribosomal Proteins

Ribosomes are essential components of the protein synthesis machinery. The process of ribosome biogenesis is well organized and tightly regulated. Therefore, any dysregulation of this process may result in aberrant cell proliferation and clinical manifestations of pathological states, such as cancer and metabolic disorders. Due to the development of proteomics techniques, quantification of ribosomal proteins provides a holistic approach to explaining the properties, functions, and distribution of ribosomal proteins, while making the ribosome a research hot spot, and ribosomal proteomics began to attract considerable attention.

As an advanced biotech company, Creative Biolabs has launched comprehensive ribosomal protein quantification analysis services to solve your problems in related research. Based on our established protein analysis platforms, our customized proteomics services have achieved remarkable goals in disease diagnosis, pathological research, and drug discovery. Thus, we are confident to provide you with the best services.

Introduction to Quantification of Ribosomal Proteins

The quantification of proteins in biological samples has been routinely used for the diagnosis of diseases and monitoring the treatment. However, large-scale protein quantification in complex samples is still a challenging task, while ribosomal proteomics attracts more attention. In addition to their canonical ribosomal functions, multiple ribosomal proteins have extra-ribosomal functions resulting in cell cycle arrest and apoptosis. Defects in ribosome biogenesis, translation, and the functions of individual ribosomal proteins, including mutations in ribosomal proteins have been linked to a diverse range of human congenital disorders termed ribosomopathies. With a deeper understanding of their biological features and roles in regulating cellular functions, maintaining cell homeostasis, and their involvement in the pathogenesis of human diseases, the quantification of ribosomal proteins provides a novel aspect to diagnosing and treating intractable diseases including cancer.

Fig. 1 In hippocampal dendrites, RP mRNAs are translated. (Fusco, Claudia M., et al., 2021)Fig. 1 RP mRNAs are translated in hippocampal dendrites.¹

Methods of Quantification of Ribosomal Proteins

As mentioned above, ribosomal proteins can provide novel insights into biological processes at the functional level, so they are very promising biomarker candidates. The quantification of ribosomal proteins in biological samples has been routinely used for the diagnosis of diseases and monitoring the treatment. Proteomics platforms are the most efficient pathway to achieve the goals involving the applications of technologies for the identification and quantification of overall ribosomal proteins' present content of a cell or tissue. In the past 20 years, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has emerged as a core tool for proteomics, particularly for the exact quantification of proteins extracted from biological samples. Recently, several advanced MS techniques, such as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS and electrospray ionization (ESI) MS, have been introduced into proteomic research.

It is no doubt that ribosomal proteins are promising in diagnosis and treatment, while the quantification of ribosomal proteins laid the basis of ribosomal proteomics analysis. Creative Biolabs offers our clients rapid and inexpensive Quantification of Ribosomal Proteins Analysis service. We are confident in presenting a comprehensive set of services to explain the secrets of ribosome proteins to worldwide clients. For more details of our service, please feel free to contact us to assist you.

Reference

  1. Fusco, Claudia M., et al. "Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins." Nature communications 12.1 (2021): 6127.
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