Protein Modification Detection

Typically, the ribosome is considered to function as just a "molecular machine" for translating mRNAs into proteins. Ribosome biogenesis and protein translation are finely coordinated and essential for cell growth, proliferation, differentiation, and animal development. Several lines of evidence suggest that the ribosome may be involved in a range of pathological processes, such as cancer, genetic diseases, and viral infection. These functions depend on a variety of proteins in the ribosome, which contains various kinds of modifications including phosphorylation, acetylation, glycosylation, methylation, and so on. As an advanced biotech company, Creative Biolabs has launched comprehensive protein modification detection services to solve your problems. Based on our established protein analysis platforms, our customized proteomics services have achieved remarkable goals in disease diagnosis, pathological research, and drug discovery.

Introduction to Protein Modification Detection

Protein modification refers to the chemical modification process of proteins during or after translation. Protein modifications increase the functional diversity of the proteome by adding functional groups such as phosphate, acetate, amido, or methyl groups to the protein and affect almost all aspects of normal cell biology and pathogenesis. Therefore, protein modification plays a key role in many cellular processes, such as cell differentiation, protein degradation, signaling, and regulatory processes, gene expression regulation, and protein interactions. With the development of ribosomal proteomics, the modification of ribosomal proteins becomes a breakthrough in solving the riddle of ribosomal protein function and provides a unique perspective for the treatment of ribosome-related diseases. With the development of precision medicine, the characteristics of protein modification, including modification categories and modification sites, are of critical importance in cell biology and disease diagnosis and prevention research.

Fig.1 Representative posttranslational modifications on proteins. (Liu, 2011)

Methods of Protein Modification Detection

With the improvement of the sensitivity and resolution of mass spectrometry technology, the sample separation and classification method was further optimized, and the sequencing depth of protein modification increased continuously. LC-MS/MS analysis can accurately determine the molecular weight of proteins or peptides, so as to detect the modification of protein or peptide sites. The characteristics of common modification are summarized in the following table.

It is no doubt that ribosomal proteins are promising in diagnosis and treatment, while the protein modification detection of ribosomal proteins provides novel insight into ribosomal proteomics analysis. Creative Biolabs offers our clients a comprehensive set of Protein Modification Detection services. For more details of our service, please feel free to contact us.

Reference

1. Liu, W. R.; et al. Synthesis of proteins with defined posttranslational modifications using the genetic noncanonical amino acid incorporation approach. Molecular BioSystems. 2011, 7(1), 38-47.