Ribosomal Protein Generation Services
Are you currently facing challenges in producing high-quality ribosomal proteins (RPs), difficulty in scaling up production, or the lack of a streamlined workflow? Our RPs generation services help you overcome these complex production hurdles and access specialized proteins through advanced recombinant DNA and expression technology. Our RPs generation services provide a specialized solution for the complex and often difficult task of producing functional RPs. These proteins are critical for a wide range of research applications, from structural biology and drug discovery to the study of ribosomopathies and cancer.
Introduction of RPs Generation Services
Ribosomes are the universal cellular factories for protein synthesis, composed of ribosomal RNA (rRNA) and approximately 80 different RPs. These proteins are not merely structural components but play crucial roles in ribosome assembly, stability, and function. The study of RPs has become a focal point in drug discovery, with many antibiotics targeting bacterial ribosomes, and a growing understanding of how mutations in human RPs are linked to diseases known as ribosomopathies and cancer.
Fig.1 Cell-free protein synthesis (CFPS) within various compartments and its regulation via gene circuits.1
Workflow
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Initial Consultation & Project Scoping
We begin with a detailed consultation to understand your project goals and requirements. This includes selecting the most suitable expression system and outlining a customized protocol. -
Gene Synthesis & Cloning
We synthesize the target gene with our proprietary codon optimization algorithm to enhance expression. The optimized gene is then cloned into the selected expression vector, ensuring a high probability of successful expression.
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Expression Optimization
Our scientists perform pilot expression tests to determine the best conditions for soluble protein production. This step is crucial for RPs, which are often prone to aggregation. We test various host strains, temperatures, and induction conditions.
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Large-Scale Expression & Purification
Once optimal conditions are identified, the project is scaled up for production. The expressed protein is then purified using advanced chromatography techniques (e.g., affinity, ion-exchange, and size-exclusion) to achieve the required purity level.
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Quality Control & ValidationThe purified protein undergoes rigorous quality control. We verify its identity and purity using techniques like SDS-PAGE and RP-HPLC. Additional characterization can be performed as needed.
Top Attractions
- Unmatched Expertise in RPs
We have deep knowledge of the unique challenges associated with these molecules, their small size, tendency to aggregate, and requirement for precise folding, and have developed robust solutions to overcome them.
- Proven Success and Efficiency
Our clients experience an average 25% increase in successful protein expression and a 30% reduction in project timelines.
- Comprehensive Workflow
Our all-in-one approach, from initial consultation and gene synthesis to final validation and quality control, ensures you receive a high-quality product with a seamless experience.
- Exceptional Customer Focus
We work closely with you at every stage, offering expert scientific guidance and maintaining clear communication to ensure your project's success.
- Risk Mitigation
Our specialized protocols and high success rate (>95%) significantly increase the probability of a positive outcome for even the most challenging projects.
Applications
- Structural Biology
Purified RPs are used to reconstitute ribosomal subunits for high-resolution studies via Cryo-EM and X-ray crystallography, providing critical insights into their structure and function.
- Drug Discovery & Screening
RPs are crucial targets for novel antibiotics and other therapeutics. Our proteins can be used in high-throughput screening assays to identify and validate potential drug candidates that modulate ribosome function.
- Biochemical & Biophysical Studies
Researchers use our proteins to investigate the dynamics of ribosome assembly, the role of individual RPs in translation, and their interactions with other molecules.
- Ribosomopathy Research
Ribosomopathies are a class of human diseases caused by defects in ribosome biogenesis or function. Our services provide a reliable source of specific mutant or wild-type RPs for disease modeling and mechanistic studies.
- Vaccine & Diagnostic Development
Viral antigens, often expressed as viral-like particles (VLPs) with RPs, can be used for vaccine development. Our services can produce these components, as well as proteins for diagnostic assays.
FAQs
Q: Why are RPs so difficult to express and purify?
A: RPs are often small, basic, and prone to misfolding and aggregation when expressed individually outside the context of the ribosome. Our specialized protocols and optimization steps are designed to overcome these inherent challenges, ensuring a successful outcome.
Q: Can your service handle both bacterial and eukaryotic RPs?
A: Yes. We offer a range of expression systems, including bacterial, mammalian, and insect cells, to ensure we can handle the unique expression and post-translational modification requirements of RPs from various species.
Q: What is the typical purity of the final protein?
A: We provide proteins with purity levels typically ranging from >90% to >95%, as determined by SDS-PAGE and RP-HPLC analysis. Purity levels are tailored to your specific application and can be further optimized upon request.
Q: Is there a risk that my protein will not be expressed?
A: We de-risk your project by offering a milestone-based payment system. While we cannot guarantee expression for every single protein, our high success rate (>95%) and extensive experience significantly increase the probability of a positive outcome.
Q: How does your service compare to purchasing catalog proteins?
A: While catalog proteins are suitable for some applications, they often lack the specificity or purity required for complex studies. Our custom service allows you to obtain the exact protein variant, tag, and quality level you need for your unique research goals, including those not available in any catalog.
Related Services
- Custom Antibody Production
We specialize in the generation of highly specific antibodies tailored to your custom-expressed recombinant proteins (RPs). These antibodies are meticulously crafted to ensure optimal performance in a wide range of immuno-related applications.
Our protein expression service utilizes advanced systems (bacterial, yeast, or mammalian) to efficiently produce high-yield, functional proteins tailored to your research needs.
We offer customized protein purification solutions, employing chromatography techniques (affinity, ion-exchange, etc.) to deliver ultra-pure, bioactive proteins.
- Protein Conjugation
At our facility, we offer comprehensive protein conjugation services to cater to your specialized in vitro assay needs. Our skilled technicians can conjugate your purified proteins with a diverse array of molecules, including fluorophores for fluorescence-based assays, enzymes for enzymatic reactions, or any other custom molecules of your choice.
We deliver highly purified, validated RPs that meet the specific needs of your project, bypassing common challenges like aggregation, low yield, and lack of solubility. Our expertise ensures you receive a high-quality product ready for immediate use in your experiments, accelerating your research timeline and de-risking your project. Contact us for more information.
Reference
- Yue, Ke, Yiyong Zhu, and Lei Kai. "Cell-free protein synthesis: chassis toward the minimal cell." Cells 8.4 (2019): 315. doi: 10.3390/cells8040315. Distributed under Open Access license CC BY 4.0, without modification.
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