Recombinant Ribosomal Protein Purification Service
Currently, researchers often encounter complex challenges when attempting to obtain high-quality recombinant ribosomal proteins (RPs) for structural or functional studies. Our recombinant ribosomal protein purification service helps you accelerate your research and obtain highly pure, active proteins through our advanced, multi-step purification platforms. We deliver not just a product, but a solution to your most complex protein purification challenges.
Fig.1 Complementary MS-based co-complex platforms to capture protein–protein interactions.1
Simple Workflow
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Project Consultation & Experimental Design
The process begins with a detailed discussion to understand your project goals, specific ribosomal protein target, and application needs. Based on this, we develop a tailored purification strategy, including host expression system selection, fusion tag selection, and a multi-step chromatography plan to achieve your desired purity and yield. -
Required Starting Materials
To initiate the service, clients typically provide a recombinant plasmid vector containing the gene for the ribosomal protein of interest, the corresponding host cell strain, such as E. coli and yeast, or a flash-frozen cell pellet from a pilot expression run.
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Expression & Cell Lysis
Following the experimental design, the ribosomal protein is expressed in the chosen host system. We then perform a gentle but efficient cell lysis to release the intracellular contents. This step is critical to prevent protein denaturation and degradation, which are common challenges when handling RPs. The lysate is clarified through high-speed centrifugation to remove cell debris and unwanted contaminants.
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Initial Affinity Chromatography
Clarified lysate is then subjected to primary affinity purification. Leveraging a highly specific affinity tag, such as His-tag, GST-tag, the target ribosomal protein is selectively captured from the complex cellular mixture. This step provides a significant initial enrichment, crucial for reducing the burden on subsequent purification stages.
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Polishing & Final Purification
To achieve the high purity required for most advanced applications, we employ a series of polishing steps. These may include ion-exchange chromatography, which separates the protein based on charge, and size-exclusion chromatography (SEC), which removes aggregates and separates the protein from any remaining contaminants, delivering a final product with exceptional homogeneity.
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Quality Control & CharacterizationThroughout the process, and especially on the final product, we perform rigorous quality control. This includes SDS-PAGE analysis to assess purity, SEC-HPLC to confirm homogeneity, and mass spectrometry for definitive protein identity verification. Endotoxin levels are also tested for projects intended for in vivo use.
Applications
- Structural Biology
Purified RPs are crucial for obtaining high-resolution 3D structures via X-ray crystallography and cryo-electron microscopy (Cryo-EM), which is vital for understanding their role in ribosome assembly and function.
- Functional Assays
These proteins are used in in vitro assays to study protein-protein or protein-RNA interactions, investigate their enzymatic activity, and analyze their role in regulating translation.
- Antibiotic Drug Discovery
RPs are key targets for new antibiotics. Our high-quality products are used in high-throughput screening and validation assays to identify and test novel therapeutic compounds.
- Antibody Development
Recombinant RPs serve as ideal antigens for generating highly specific antibodies for research tools or diagnostic applications.
Why Pick Us Over Others?
- High Purity & Yield Guaranteed
Our proprietary chromatography and filtration techniques ensure >95% purity of recombinant RPs, with optimized protocols to maximize recovery rates. Ideal for structural biology, drug discovery, and functional assays.
- Customizable Purification Strategies
Tailored solutions for your specific protein-whether you need native folding, tag removal, or endotoxin-free preparations. We adapt to your downstream applications, from X-ray crystallography to enzyme activity studies.
- Scalable Production for Any Project Size
From milligram-scale pilot batches to gram-level manufacturing, our platform supports small-scale R&D to large-scale industrial applications. Flexible scaling ensures cost-efficiency at every stage.
- End-to-End Quality Control
Every batch undergoes rigorous testing via SDS-PAGE, HPLC, and mass spectrometry to verify integrity, activity, and contamination-free results. Detailed reports are included with every delivery.
FAQs
Q: What types of starting materials do you accept for the service?
A: We accept a variety of starting materials, including purified plasmid DNA encoding your ribosomal protein, bacterial or yeast cell lines ready for expression, or prepared cell pellets from your own expression trials. We are flexible and can work with you to determine the best starting point for your project.
Q: What is the typical purity of the final product?
A: The purification protocol was optimized to achieve >95% final purity, confirmed by SDS-PAGE analysis. For more demanding applications like structural biology, we can deliver products at greater than 98% homogeneity, ensuring your experiments are not compromised by impurities.
Q: How is your service different from purifying the entire ribosome?
A: Our service focuses on the purification of a single, specific recombinant ribosomal protein, which is essential for studying its individual function or structure. In contrast, whole ribosome purification isolates the entire complex, which may not be suitable for applications that require a single, pure protein component.
Q: Can you handle proteins from both prokaryotic and eukaryotic sources?
A: Yes, our expertise extends to expressing and purifying RPs from both prokaryotic and eukaryotic systems. Our platform and protocols are adapted to the specific characteristics of each host.
Q: What quality control measures do you have in place to ensure protein integrity?
A: We use a multi-faceted approach to quality control. In addition to standard purity analysis via SDS-PAGE, we employ mass spectrometry for definitive identity confirmation and SEC-HPLC to check for aggregation and ensure the protein is in its native, functional form.
At Creative Biolabs, we are committed to providing high-quality, reliable, and scientifically sound solutions to accelerate your research. Our recombinant ribosomal protein purification service is a testament to our expertise and dedication.
Reference
- Meyer, Margaux De, et al. "Keeping in touch with type-III secretion system effectors: mass spectrometry-based proteomics to study effector–host protein–protein interactions." International journal of molecular sciences 21.18 (2020): 6891. DOI: 10.3390/ijms21186891. Distributed under Open Access license CC BY 4.0, without modification.
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