Recombinant Ribosomal Protein Expression Service
Are you presently encountering difficulties in acquiring top-tier, functional ribosomal proteins (RPs) for your research endeavors, or grappling with the intricacies of RPs expression and purification? Our recombinant RPs expression service helps you streamline your research and obtain high-quality, functionally active proteins through advanced recombinant DNA technology and a specialized purification platform. We help you overcome expression bottlenecks and achieve your scientific goals with precision and efficiency.
Service Profile
RPs play a critical role in translation, and their dysfunction is linked to a variety of diseases. The production of high-quality recombinant RPs is essential for research into ribosome structure, function, and drug discovery. Our services are designed to address the challenges associated with expressing these unique proteins, providing researchers with the reliable tools needed to advance their understanding of this complex biological process.
Fig.1 The key steps in the expression of heterologous protein in yeast cells.1
Task Flow
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Gene Synthesis & Vector Construction
We begin by optimizing the gene sequence for the chosen host expression system, such as E. coli or mammalian cells, to maximize protein yield. The optimized gene is then synthesized and cloned into an appropriate expression vector. -
Small-Scale Expression & Optimization
A pilot expression run is performed to identify the optimal conditions for the target protein. We systematically screen different variables, such as induction temperature, induction time, and media composition, to maximize solubility and expression.
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Large-Scale Expression & Purification
Once optimal conditions are determined, the process is scaled up for large-volume expression. The cells are harvested, and the target protein is purified using a combination of chromatographic techniques, such as affinity, ion-exchange, and size-exclusion chromatography, to achieve the desired purity level.
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Quality Control & CharacterizationThe purified protein undergoes rigorous quality control. Protein identity, purity, and integrity were verified through comprehensive analysis. This may include SDS-PAGE, Western blotting, and mass spectrometry.
Why Opt for Our Services?
Choosing Creative Biolabs for your recombinant RPs expression needs means partnering with a team of experts dedicated to quality and reliability. Our deep expertise in this specialized field, combined with our rigorous quality control processes, ensures that you receive high-purity, functionally active proteins every time. Published Data from our projects consistently show high success rates and reproducible results, which are critical for advancing research.
Applications
- Structural Biology
Used for X-ray crystallography, cryo-electron microscopy (Cryo-EM), and NMR to determine the atomic-level structure of the ribosome and its interactions with other molecules, such as antibiotics.
- Drug Discovery
Serves as a target for screening small molecules and compounds that modulate ribosome function, which is a critical approach in the development of new antibiotics.
- Biochemical and Biophysical Studies
Enables a deeper understanding of the functional roles of individual RPs in translation, ribosome assembly, and other cellular processes.
- Immunological Research
It can be used to generate antibodies for diagnostic kits and research, and to study the immune response to ribosomal components in certain autoimmune diseases.
FAQs
Q: What expression systems are available for your recombinant RPs service?
A: We primarily utilize advanced E. coli expression systems for their efficiency and cost-effectiveness. However, we can also explore other systems, such as mammalian or insect cells, based on the specific requirements of your protein and project goals. We invite you to contact our technical team to discuss the best system for your needs.
Q: How do you ensure the quality and purity of the expressed RPs?
A: Our quality assurance process is rigorous. Each purified protein undergoes multiple checks, including SDS-PAGE for purity, Western blotting for identity, and often mass spectrometry for confirmation. A detailed Certificate of Analysis (CoA) is provided to document these results, ensuring transparency and reliability.
Q: Can you handle the expression of large or challenging RPs?
A: Yes, our platform is optimized to tackle challenging protein targets. We employ specialized expression vectors and purification protocols, and our team has extensive experience in overcoming issues like low solubility, aggregation, and toxicity often associated with complex RPs.
Q: Do you offer any post-expression services, such as protein modification or functional assays?
A: We do! Beyond basic expression and purification, we offer a range of complementary services, including protein labeling, protein-protein interaction studies, and more. Our team is happy to discuss your specific requirements and how we can support your downstream applications.
Creative Biolabs is your trusted partner for high-quality, reliable recombinant RPs expression. Our specialized expertise, advanced platforms, and commitment to customer success ensure that your project receives the attention and precision it deserves. By partnering with us, you can overcome expression challenges and accelerate your scientific discoveries!
Reference
- Popova, Larissa G., et al. "Yeast heterologous expression systems for the Study of Plant Membrane Proteins." International Journal of Molecular Sciences 24.13 (2023): 10768. DOI: 10.3390/ijms241310768. Distributed under Open Access license CC BY 4.0, without modification.
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