Protein Interaction Analysis Service

The eukaryotic ribosome is the cellular translational machinery primarily responsible for protein synthesis from mRNA and consists of four rRNA species and 79 ribosomal proteins. The production of this machinery called ribosome biogenesis is an extraordinarily complex process involving all three RNA polymerases and more than 150 non-ribosomal factors that are required for the synthesis, processing, transportation, and assembly of pre-ribosomes. Therefore, the interaction analysis of ribosomal proteins is a promising approach to uncovering the functions of ribosomal associated proteins. As an advanced biotech company, Creative Biolabs has launched comprehensive protein interaction analysis services to solve your problems. Based on our established protein analysis platforms, our customized proteomics services have achieved remarkable goals in disease diagnosis, pathological research, and drug discovery.

Introduction to Protein Interaction Analysis

Proteins can function as independent molecules or interact with other proteins to form functional complexes. The potential combinations of proteins that form complexes are enormous. For example, biogenesis of the large ribosomal subunit requires the coordinate assembly of two rRNAs and 33 ribosomal proteins, while the analysis of protein-protein interaction can explain how this complex process happens. Therefore, it is necessary to investigate experimentally what combinations form protein complexes. Furthermore, it is also important to examine details, such as affinity and kinetic properties of protein-protein interactions, to understand the complicated biological processes in which they participate.

Methods of Protein Interaction Analysis

The research field of protein-protein interactions is an interdisciplinary and specialized field that spans all aspects of biology, physics, and chemistry. Therefore, it is obvious that progress in fields requiring equipment based on sophisticated principles such as protein interaction research is dependent on the discovery and utilization of principles and phenomena in physics and chemistry. Nowadays, multiple methods are utilized to confirm protein-protein interactions including pull-down assay, two-hybrid assay, gel filtration chromatography, isothermal titration calorimetry (ITC), fluorescence resonance energy transfer (FRET), luminescent oxygen channeling assay (LOCI), reflectometric interference spectroscopy (RIFS), surface plasmon resonance spectroscopy (SPR), circular dichroism spectroscopy (CD), raman optical activity spectroscopy (ROA), small angle X-ray scattering (SAXS), nuclear magnetic resonance spectroscopy (NMR), and cryo-electron microscopy (Cryo-EM). The characteristics of each method are summarized in the following table.

Table.1 Comparation of common protein interaction analysis methods.

+: possible and suitable, ±: possible depending on conditions, -: rather unsuitable or impossible.

It is no doubt that ribosomal proteins are promising in diagnosis and treatment, while the protein interaction analysis of ribosomal proteins provides novel insight into ribosomal proteomics analysis. Creative Biolabs offers our clients Protein Interaction Analysis service. We are confident in presenting a comprehensive set of services to explain the secrets of ribosome proteins to worldwide clients. For more details of our service, please feel free to contact us to assist you.