Golgi Apparatus Isolation Service
Is your current organelle isolation protocol both time-consuming and producing suboptimal purity results? Is your research hampered by cross-contamination from traditional methods, leading to unreliable downstream data? Our specialized Golgi apparatus isolation protocol ensures high-yield recovery of structurally intact Golgi membranes with preserved native protein complexes. You can expect to receive highly pure, functionally intact Golgi fractions, essential for sensitive downstream applications like proteomics, lipidomics, and functional assays. Our service delivers a level of consistency and purity that is difficult to achieve with in-house protocols, ensuring your data is reliable and reproducible.
Service Highlights
The Golgi apparatus is a dynamic and essential organelle central to a eukaryotic cell's secretory and endocytic pathways. It functions as a sorting and modification hub, processing proteins and lipids destined for various cellular locations or secretion. Dysfunctional Golgi morphology and its associated proteins have been linked to a wide range of human disorders, including neurodegenerative diseases and cancer. To study this organelle and its role in pathology, the precise isolation of the Golgi apparatus is a critical first step. Our service leverages advanced techniques to provide a pure and intact Golgi fraction, enabling researchers to explore this vital organelle with unparalleled accuracy.
Fig.1 Signaling pathways play an essential role in organizing the Golgi apparatus and maintaining neuronal polarity during neurogenesis.1
Streamlined Workflow
-
Sample Collection & Homogenization
Your provided samples are processed in a proprietary, gentle homogenization buffer. This crucial first step ensures the Golgi apparatus remains largely intact while the cellular membrane is disrupted. -
Organelle Fractionation & Isolation
Using a combination of differential centrifugation and our proprietary spin-column technology, we separate the Golgi membranes from other organelles and cellular debris. This rapid, multi-stage process minimizes the risk of cross-contamination that plagues single-step methods.
-
Purity and Integrity Verification
Isolated fractions are rigorously tested for purity. We use marker protein analysis (e.g., Western blotting for Golgi-specific markers and a lack of markers for contaminating organelles like mitochondria or the ER) and enzyme activity assays to confirm the quality of the isolation.
-
Protein and Lipid Extraction
The purified Golgi fractions undergo subsequent processing for sequential extraction of proteins and lipids. This step is tailored to your specific downstream analysis needs, such as preparation for mass spectrometry.
-
Final Quality Control & DeliveryAll samples and data are subjected to a final quality check before being shipped to you with a comprehensive report.
Final Deliverables
- Purified Golgi Apparatus Sample
A high-quality, intact sample ready for your specific application.
- Purity Analysis Report
A detailed report with Western blot data and marker enzyme activity results confirming the purity of your sample.
- Comprehensive Project Report
A full report detailing the methods used, quality control data, and an overview of the project's outcomes.
Why Choose Us?
Creative Biolabs stands apart by offering a service built on precision, efficiency, and a deep understanding of cellular biology. Our use of advanced spin-column technology eliminates the need for cumbersome ultracentrifugation, resulting in faster processing times and samples that are preserved in their native state. This approach guarantees minimal cross-contamination, which is essential for obtaining accurate and reliable data in sensitive downstream analyses like proteomics and lipidomics. Our expertise in sub-cellular analysis ensures that every project is handled with the utmost scientific rigor.
FAQs
Q: Is your method suitable for isolating the Golgi from different sample types?
A: Yes, our service platform is engineered for maximal adaptability across diverse experimental paradigms. We have optimized protocols for a wide range of samples, including cultured cells, various animal tissues, and even plant material. Please contact us to discuss your specific sample type, and we can tailor a plan to meet your needs.
Q: How do you ensure the isolated Golgi samples are not contaminated with other organelles?
A: Our multi-stage purification strategy employs proprietary spin-column technology integrated with a precision-engineered workflow to achieve organelle-specific separation of the Golgi apparatus from contaminating cellular structures. We then confirm the purity using well-established biochemical markers and methods like Western blotting to guarantee a high-quality sample.
Q: How does your service outperform traditional in-house density gradient centrifugation for Golgi apparatus isolation?
A: Our service bypasses the need for expensive ultracentrifuges and reduces the risk of sample contamination and degradation. It is significantly faster and provides a higher purity yield, which translates to more reliable and reproducible data for your downstream experiments.
Q: What kind of downstream analysis can I perform with the isolated Golgi fractions?
A: Our high-purity fractions are ideal for a variety of applications. You can use them for mass spectrometry-based proteomics and lipidomics, western blot analysis, enzyme activity assays, and functional studies of protein and lipid trafficking. The possibilities are vast!
Q: What is the typical turnaround time, and how does the cost compare to traditional methods?
A: The timeframe can vary depending on your specific project, but our streamlined process ensures efficiency. While the initial cost may seem higher than reagent-based in-house methods, the saved time, reduced risk of failed experiments, and reliable data quality often result in a lower overall project cost.
At Creative Biolabs, our Golgi apparatus isolation service provides a professional, reliable, and efficient solution for researchers aiming to study this critical organelle. Through integration of state-of-the-art technology and stringent quality assurance protocols, we provide ultra-pure, structurally preserved Golgi fractions that empower transformative discoveries across cell biology, biochemistry, and disease research. Ready to accelerate your research with our Golgi apparatus isolation service? Contact our expert team today!
Reference
- Mohan, Aurel George, et al. "The Golgi apparatus: A voyage through time, structure, function and implication in neurodegenerative disorders." Cells 12.15 (2023): 1972. DOI: 10.3390/cells12151972. Distributed under Open Access license CC BY 4.0, without modification.
(USA)
(UK)
(Germany)
